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, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Circular RNAs;
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variation, transcriptomics analysis, epigenetic variation, identification of imprinted genes, noncoding RNAs (circular RNAs), genome editing;
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MicroRNAs constitute a particularly important class of small RNAs given their abundance, broad phylogenetic conservation and strong;
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PIWI-interacting RNAs (piRNAs) are the third and most-recently discovered group of silencing-inducible small RNAs in animals. PIWI;
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alter guide RNA stability, specificity, and efficiency, as well as to create inducible guide RNAs, append additional functional domains, and;
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and authoritative, RNAi and Small Regulatory RNAs in Stem Cells: Methods and Protocols aims to accelerate progress in this crucial field by;
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to study short RNAs expressed at a low level and model systems that are particularly suitable to investigate specific aspects of endo-siRNAs;
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for cancer DNA and circulating tumour cells, genomic analysis, mi-RNAs, and proteins. Written in the highly successful Methods in Molecular;
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include methods and techniques for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs such as small;
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comprehensive, RNA Detection: Methods and Protocols is a valuable resource for novel and experiences scientist in the expanding field of RNAs.;
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The discovery of catalytic RNAs in the mid-1980s marked the beginning of a new era in RNA biology and an ever increasing appreciation;
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historical importance and the importance of researchers using bioassay, quantification, and characterization of cytokine related RNAs;
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importance and the importance of researchers using bioassay, quantification, and characterization of cytokine related RNAs, posttranscriptional;
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of exosomes and other secreted vesicles, as well as micro and non-coding RNAs, that have been demonstrated to control the tuning of the in vivo micro;
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expression and enzyme activity of ecto-ARTCs; HPLC-based enzymes assays for Sirtuins; and identifying target RNAs of PARPs. Written in the highly;
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for the enrichment, separation and purification of these novel RNAs. Building up from general principles of RNA biochemistry and biophysics;
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small RNAs and natural antiviral RNAi responses as well as for the development and optimization of RNAi-based antiviral drugs. As RNAi is a;
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the identification and quantification of non-coding RNAs, including miRNAs, tasiRNAs, phasiRNAs, lariat originated circRNAs and back-spliced;
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-coding RNAs and secondary structures; genome-wide mining of disease resistance gene analogs; genome editing, and bioactive compound and;
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. This volume covers research methods in riboswitch discovery and validation, synthesis and sample prep methods for large RNAs, riboswitch;
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of discovering non-coding RNAs (ncRNAs) which spans through a major portion of the genome. These RNAs most often act as riboregulators, and actively;
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interactions and induction of innate immune responses. Subsequent chapters outline diverse methods to detect small interfering RNAs, microRNAs, and;
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unpublished troubleshooting hints. They cover all modern techniques for the handling, analysis and modification of RNAs and their complexes with;
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unpublished troubleshooting hints. They cover all modern techniques for the handling, analysis and modification of RNAs and their complexes with;
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. This volume describes strategies for the discovery and validation of small RNAs. It provides a snapshot of our current understanding of the;
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