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Methods for Affinity-Based Separations of Enzymes and Proteins

short introduction and illustrative protocols for some important and current affinity-based approaches inthe area ofdownstream;

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Protein Purification

Protein purification is vital for the characterisation of the function, structure and interactions of the protein of interest. This book;

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Affinity Membranes

separations, the formation processes of semi-permeable membranes and the kinetics of separations based on affinity membranes.;

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Molecular Design and Modeling: Concepts and Applications, Part A: Proteins, Peptides, and Enzymes

Computer-based design and modeling, computational approaches, and instrumental methods for elucidating molecular mechanisms of protein;

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Single-Stranded DNA Binding Proteins

affinity and specificity. SSBs also aid genome metabolic processes through direct interactions with key proteins in genome maintenance enzymes;

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Protein Purification

immobilized pH gradients * Affinity ligands from chemical and biological combinatorial libraries * Membrane separations * Refolding of inclusion;

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Methods for fragments screening using Surface Plasmon Resonance

This volume describes methods and protocols for the fragment-based screening of proteins using Surface Plasmon Resonance (SPR). The initial;

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Fe-S Cluster Enzymes Part A

reporters to track Fe-S cluster assembly and transfer reactions, Mechanism-based strategies for structural characterization of radical SAM reaction;

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CRISPR-Cas Enzymes

within CRISPR complexes, Fluorescence-based methods for measuring target interference by CRISPR-Cas systems, Native State Structural;

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High-Performance Liquid Chromatography of Peptides and Proteins

approach to HPLC with minimal theory, although the underlying principles for peptide and protein separations are clearly expressed. All of the;

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Bioseparations Engineering

Multidisciplinary resource for graduate studies and the biotechnology industry Knowledge of the genetic basis of biological functioning;

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Advances in Biomolecular EPR

Sites in the Prion and Related Proteins, The catalytic role of metal-radical/protein-based radicals in heme enzymes, Rigid Cu2+-based spin;

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Practical High-Performance Liquid Chroma

This textbook aims to present the knowledge which is necessary for everyday HPLC measurements to be made, in a detailed and easy-to-follow;

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Fe-S Cluster Enzymes Part B

-sulfur enzymes, NRVS for Fe in biology and its experiment and basic interpretation, methods for studying iron regulatory protein 1, an;

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Capillary Electrophoresis of Proteins

optimizing CE techniques in the laboratory. Includes separation methods bases on mass, charge, isoelectric point, molecular sieving, and affinity;

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Protein Interactions: Computational Methods, Analysis and Applications

affinity of complexes, and study the influence of mutations on diseases. Protein interactions are also crucial in structure-based drug design.This;

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Single-Molecule Studies of Nucleic Acids and Their Proteins

, etc.). It then exemplifies the use of these various methods in the study of three families of DNA enzymes: polymerases, helicases and;

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Isolation and Purification of Proteins

-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial;

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Isolation and Purification of Proteins

-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial;

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Centrifugal Separations in Biotechnology

, including batch and semi-batch centrifuges, disk-stack and tubular decanter centrifuges; methods for increasing solids concentration; laboratory and;

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Advances in GAPDH Protein Analysis

This book presents modern and classic analytical approaches that are crucial for the biochemical and functional characterization of the;

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DNA Repair Enzymes: Structure, Biophysics, and Mechanism

focuses on DNA Repair Enzymes. Topics in this updated volume include MacroBac: New Technologies for Robust and Efficient Large-Scale Production;

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NanoArmoring of Enzymes: Rational Design of Polymer-Wrapped Enzymes

series that focuses on nanoarmoring of enzymes and the rational design of polymer-wrapped enzymes. This new volume presents the most updated;

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Modern Approaches in Drug Discovery

Imaging, Target ID using chemical probes, Mining the microbiome for targets, Data driven approaches for diversity and drug-likeness, Affinity;

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Trace and Ultratrace Analysis by HPLC

also highlights the techniques required for a quality control program and such advanced technology as mass spectrometry. 1990 (0 471-82584-0;

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Methods in Protein Structure & Stability Analysis

commonly found in proteins, and serve as enzymes, structural elements, hormones, immunoglobulins, etc., and are involved throughout the body, and;

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Microbial Enzymes and Biotechniques

, bioengineering of enzymes for bioactive compounds, the production of hydrolytic and oxidative enzymes from plant raw materials, and prebiotics and;

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